Yes. None of the cards specify the gender or sexual orientation of the players, or of the people they have f’d. In fact, words like “male”, “female”, “gay”, “straight”, “same sex” and “opposite sex” do not occur anywhere in the f’ing deck.
Cards DO, however, specify the kinds of genitals players have encountered in their sexual rendezvous (eg: "Have you ever given oral sex to a person with a vagina?"). Our f’ing scientists have determined that this method allows for maximum fun and playability. IF, HOWEVER, it does not sound fun to you to acknowledge in a group whether any of your former partners have had penises/vaginas, you might not think TFT is fun.