Using directed evolution to improve luminosity
Some of you are probably wondering how we are working to improve the luminosity of our plant. We've already discussed some of the ways we are doing this (by testing different DNA sequences, eg promotors and codons) but here's a video from Jamey talking about how he's using Directed Evolution to improve the underlying genes:
Here are some pictures which illustrate what's going on. The first picture shows the colony's. As you can see not all of them are glowing as some of the mutations are negative:
We then put the same plate in our luminometer and read the light intensity, as you can see there's variation between them with colony 1 showing about 10% improvement on the others. We can then analyse what those variations are, and improve the luminosity.